磷酸酶活性分析试剂盒Phosphatase Assay kit现货

英文名：Phosphatase Assay kit

储存：冷藏（2-8℃）

规格：1000次/盒

品牌：子科生物ZIKER

本产品的详细报价、说明书、规格、品牌、技术参数、使用操作可以咨询子科生物在线销售人员QQ：2811391951或者电话联系:0755-66609091.

运输和保存：Shipped at ambient temperature. Upon arrival, store at 4°C. The substrate is light sensitive and should be protected from direct sunlight or UV sources. 

产品介绍：

The Phosphatase Assay kit is designed to measure the activity of phosphatases in biological samples and to screen for agonists and inhibitors of phosphatases. The Phosphatase Assay kit uses para-nitrophenyl phosphate (pNPP), a chromogenic substrate for most phosphatases,including alkaline phosphatases, acid phosphatases, protein tyrosine phosphatases and serine/threonine phosphatases (Figure 1). The phosphatases remove the phosphate group to generate p-nitrophenol, which is deprotonated under alkaline conditions to produce pnitrophenolate that has strong absorption at 405nm. The kits components are sufficient for performing up to 1000 assays in 96-well plate format and easily adaptable to cuvettes or 384-well plates. 

磷酸酶活性分析试剂盒Phosphatase Assay kit现货

Equilibrate the substrate and assay buffer to room temperature before use.

1. Make appropriate serial dilutions of your test samples with PA Assay Buffer. Prepare enough solution to perform

assays in triplicate.

2. Transfer 50μl diluted test samples to wells in a 96-well plate. In addition, use 50μl PA Assay Buffer without

enzyme for blank wells.

3. Start the reaction with the addition of 50μl PA Substrate to each well.

4. Incubate for 10-30 minutes at room temperature.

NOTE: For dynamic assay, measure the absorbance every minute at 405nm.

5. At the end of the incubation, add 50μl Stop Solution to each well to stop the reaction.

6. Measure the absorbance at 405nm. 

深圳子科生物还同时经营ELISA酶联免疫检测试剂盒、分子生物学检测试剂盒、免疫学检测试剂盒、各品牌原装进口抗体抗原、细胞菌株、生物培养基实验耗材等多门类上万余种科研试剂产品，如有其它需要欢迎来电咨询洽谈0755-66609091。

磷酸酶活性分析试剂盒Phosphatase Assay kit现货

相关产品：

786-562Phenol Red Solution [0.5%]125ml

786-563Phenol Red Solution [0.5%]250ml

786-564CTAB Extraction Solution60ml

786-565CTAB Extraction Solution125ml

786-566Gram Negative Lysis Buffer125ml

786-567Gram Negative Lysis Buffer250ml

786-568STE Buffer [10X] (100mM Tris.HCl (pH8.0), 10mM EDTA, 1M NaCl)500ml

786-569STE Buffer [10X] (100mM Tris.HCl (pH8.0), 10mM EDTA, 1M NaCl)1 Liter

786-571Bicinchoninic Acid (BCA) Protein Assay1,000 Assays/ 5,000 Micro-assays

786-573Bicinchoninic Acid (BCA) Protein Assay: Reducing Agent Compatible250 Assays

786-574Blue Dextran Loading Dye10ml

786-575Ponceau S Stain; 0.1% 250ml

786-576Ponceau S Stain; 0.1% 500ml

786-579Gram Negative Lysis Buffer500ml

786-580Gram Negative Lysis Buffer1L

786-581Avidin5mg

786-582Avidin25mg

786-583Avidin100mg

786-584Streptavidin5mg